Disruption of RNA editing in Leishmania tarentolae by the loss of minicircle-encoded guide RNA genes.
نویسندگان
چکیده
منابع مشابه
The mitochondrial RNA ligase from Leishmania tarentolae can join RNA molecules bridged by a complementary RNA.
A biochemical characterization was performed with a partially purified RNA ligase from isolated mitochondria of Leishmania tarentolae. This ligase has a K(m) of 25 +/- 0.75 nM and a V(max) of 1.0 x 10(-4) +/- 2.4 x 10(-4) nmol/min when ligating a nicked double-stranded RNA substrate. Ligation was negatively affected by a gap between the donor and acceptor nucleotides. The catalytic efficiency o...
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The molecular mechanism of RNA editing in trypanosomatid mitochondria is an unsolved problem. We show that two classes of ribonucleoprotein complexes exist in a mitochondrial extract from Leishmania tarentolae and appear to be involved in RNA editing. The 'G' class of RNP complexes consists of 170-300 A particles which contain guide RNAs and proteins, show little terminal uridylyl transferase (...
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In vitro RNA editing-like activity in a mitochondrial extract from Leishmania tarentolae.
A mitochondrial extract from Leishmania tarentolae directs the incorporation of uridylate (U) residues within the pre-edited domain of synthetic cytochrome b (CYb) and NADH dehydrogenase subunit 7 mRNA. This has several characteristics of an in vitro RNA editing activity, but no direct evidence for involvement of guide RNAs was obtained. Inhibition by micrococcal nuclease suggests a requirement...
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a Howard Hughes Medical Institute, UCLA School of Medicine, Los Angles, CA 90095-1662, USA b Laboratory of Protein Crystallography and Structural Biology, Physics Institute of San Carlos, Uni6ersity of Sao Paulo, A6. Dr. Carlos Botelho 1465, P.O. Box 369, San Carlos, SP 13560-970, Brazil c Department of Microbiology, Immunology and Molecular Genetics, 6780 MacDonald Building, 675 Charles Young ...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 1994
ISSN: 0261-4189
DOI: 10.1002/j.1460-2075.1994.tb06907.x